Research Paper Volume 11, Issue 23 pp 11202—11223

ciRs-6 upregulates March1 to suppress bladder cancer growth by sponging miR-653

Figure 4. miR-653 rescues the tumor suppressive effect of ciRs-6 in bladder cancer. (A, B) qPCR was used to detect the levels of ciRs-6, SLC41A2 and miR-653 in each group; (C, D) CCK8 assays were performed to evaluate cell viability; (EH) clone formation assays were used to evaluate clone forming ability; (IL) cells in S phase were assessed by cell cycle analysis; (MO) mouse subcutaneous tumor model experiments were used to detect in vivo tumorigenic effects. Each group contained 5 mice. The results are displayed as the mean±SEM, *p<0.05, **p<0.01.