Research Paper Volume 11, Issue 23 pp 11202—11223

ciRs-6 upregulates March1 to suppress bladder cancer growth by sponging miR-653

Figure 6. ciRs-6 suppresses bladder cancer growth by elevating March1 levels. Three siRNAs for March1 were designed and transfected into ciRs-6 overexpressed bladder cancer cells. (A) qPCR was used to detect the level of March1 in each group; (B, C) CCK8 assays were performed to evaluate cell viability; (DF) clone formation assays were used to evaluate clone forming ability; (GI) cells in S phase were assessed by cell cycle analysis in each group. The results are displayed as the mean±SEM, *p<0.05, **p<0.01.