Figure 3. Analysis of the subclones and evolution of primary and recurrent GBM samples from patient NO. 05. (A) Four intra-tumor loci from the primary tumor and 5 intra-tumor loci from the recurrent tumor from the same patient were sequenced and analyzed. (B) VAF distribution of different subclone types at each intratumor locus. (C) A clonal evolution map of each intratumor locus. (D) Clonal structure distribution of each intratumor locus. (E) A tumorigenic chart of intratumor loci.