Research Paper Volume 11, Issue 23 pp 11268—11313

The RXFP3 receptor is functionally associated with cellular responses to oxidative stress and DNA damage

Figure 5. Initial analysis of the unique RXFP3 interactomes under stress conditions indicates a role in DNA damage response and cell cycle control. (A) VennPlex analysis of the different RXFP3 interactomes, RXFP3 without stress (control; orange) (n=3), RXFP3 in response to Oxidative stress using 100 nM hydrogen peroxide for 90 minutes (Ox Stress; Green) (n=3), and DNA damage using 1 μM of camptothecin for 3 hours (DNA damage; blue) (n=3). For further analysis, the proteins unique to Oxidative stress and DNA damage were used for further analysis using Textrous! (textrous.irp.nia.nih.gov), which employs latent semantic indexing to achieve an easy and unbiased appreciation of our data, supplying a list of words and noun-phrases related to our dataset. Further analysis of these wordlists using WriteWords (http://www.writewords.org.uk), enabled us to count the most prominently present (B) words, and (C) noun-phrases associated to the specific datasets, from this it becomes clear that RXFP3 interacts with proteins involved in DNA damage response (Oxidative Stress) and Cell cycle control (DNA damage) in response to stress. Next, we extracted the hierarchical wordcloud from Textrous!, where we see the specific words for (D) Control, indicating a role in control of neurodegenerative disorders such as Alzheimer’s disease and frontotemporal dementia (FTD), (E) Oxidative Stress, again indicating an association with DNA damage repair; and (F) DNA damage, indicating a connection to cell cycle control.