Research Paper Volume 11, Issue 23 pp 11314—11328

CircPTPRA acts as a tumor suppressor in bladder cancer by sponging miR-636 and upregulating KLF9

Figure 3. CircPTPRA inhibits the proliferation of BC cells. (A) Design of siRNAs targeting circPTPRA. (B, C) Expression of circPTPRA in BC cell lines after siRNAs treatment and lentivirus transfection. (DF) Effects of circPTPRA silencing on BC cell proliferation measured through CCK-8 and colony formation assays. (GI) Effects of circPTPRA overexpression on BC cell proliferation detected by CCK-8 and colony formation assays. Data are presented as the mean ± SD of three experiments. *P< 0.05, **P< 0.01(Student’s t-test).