Figure 4. CircPTPRA acts as a sponge of miR-636. (A) Predicted miRNA target of circPTPRA identified on circInteractome and circBank. (B, C) Expression of circPTPRA detected by qRT-PCR analysis and gel electrophoresis of samples obtained by RNA pull-down assay. (D, E) Analysis of miRNAs bound to circPTPRA and control probes, measured by qRT-PCR. (F) Luciferase reporter assay results demonstrating the interaction between circPTPRA and miR-636. (G) Enrichment of circPTPRA captured by biotin-coupled miR-636 mimic or its mutant variant, as detected by qRT-PCR. (H) Co-localization of circPTPRA and miR-636 determined by FISH assay. Scale bar=50μm. Data are presented as the mean ± SD of three experiments. *P < 0.05, **P < 0.01(Student’s t-test).