Figure 3. Effect of circAMOTL1 overexpression on PAX treatment. MDA-MB-231 cells stably transfected with vector control or circAMOTL1 were treated with 1 μg/ml PAX for 24 hours. (A) Cells morphological changes upon PAX treatment. (B) Cell apoptosis upon PAX treatment. Cells were stained with Annexin V/PI double staining followed by FACS analysis. Experiments were performed in triplicate. (C) Quantitation of double negative cells (alive cells). (D) Cell invasive ability upon PAX treatment was measured with Matrigel invasion assay. Experiments were performed in triplicate. **p<0.01 compared to untreated vector control. ##p<0.01 compared to PAX treated vector control (n=3).