Research Paper Volume 11, Issue 23 pp 11463—11473

LncRNA EPIC1 downregulation mediates hydrogen peroxide-induced neuronal cell injury

Figure 2. Ectopic overexpression of Lnc-EPIC1 inhibits H2O2-induced neuronal cytotoxicity. Stable SH-SY5Y cells with the lentiviral Lnc-EPIC1-expression construct (three lines, “Lnc-EPIC1-OE-1/-2/-3”) or the vector control cells (“Vector”) were treated with hydrogen peroxide (H2O2, 300 μM), cells were further cultured for indicated time, expression of Lnc-EPIC1 (A) and listed mRNAs (B and C) were tested by qPCR assay; Cell survival (by the CCK-8 assay, D), death (by the LDH assay, E) and apoptosis (by the caspase-3 activity, ssDNA ELISA and JC-1 staining assays, FH) were tested. The primary human neuron cultures were infected with the lentivirus with Lnc-EPIC1 construct (“LV-Lnc-EPIC1”) or empty vector (“Vector”) for 48h, treated with hydrogen peroxide (H2O2, 300 μM) for applied time, Lnc-EPIC1 expression (I), neuronal survival (by the CCK-8 assay, J) and death (by the LDH assay, K) were tested. Bars stand for mean ± standard deviation (SD, n=5). * P < 0.05 vs. “Ctrl” treatment of “Vector” cells. #P < 0.05 vs. H2O2 treatment of “Vector” cells. Experiments in this figure were repeated three times, and similar results were obtained. Bar= 100 μm (H).