Research Paper Volume 11, Issue 23 pp 11463—11473

LncRNA EPIC1 downregulation mediates hydrogen peroxide-induced neuronal cell injury

Figure 3. Lnc-EPIC1 siRNA potentiates H2O2-induced neuronal cell death. SH-SY5Y cells (AH) or the primary human neuron cultures (IK) were transfected with applied Lnc-EPIC1 siRNA (“EPIC1-siRNA1/2”, 100-500 nM) or scramble control siRNA (“scr-siRNA”, 500 nM) for 48h, then treated with/without hydrogen peroxide (H2O2, 300 μM), cells were further cultured for indicated time, expression of Lnc-EPIC1 (A and I) and listed mRNAs (B and C) were tested by qPCR assay; Expression of listed proteins was tested by Western blotting assay (D); Cell viability (by the CCK-8 assay, E and J), cell death (by the LDH assay, F and K) and apoptosis (by the caspase-3 activity and ssDNA ELISA, G and H) were tested. Listed proteins were quantified, with the values normalized to Tubulin (D). Bars stand for mean ± standard deviation (SD, n=5). * P < 0.05 vs. “Ctrl” treatment in “scr-siRNA” cells. #P < 0.05 vs. H2O2 treatment of “scr-siRNA” cells. Experiments in this figure were repeated three times, and similar results were obtained.