Figure 3. RSV regulates mitochondrial function in aged oocytes. (A) After treatment with RSV, the oocytes were stained with JC-1. Mitochondria that had high MMP were stained red while mitochondria that had low MMP were stained green. Bar = 100 μm. (B) Quantitative analysis of MMP in (A). (C) The effect of RSV on mtDNA copy numbers. The relative mtDNA copy numbers were detected by real time qPCR and normalized to the amount of β-globin. (D) The effect of RSV on ATP production. ATP level was detected as described in the material and method section. All the experiments were conducted in triplicate, and the relative expression data were normalized to embryo numbers per sample. Data are presented as means ± S.E.M of three independent experiments. Different lowercase letters represent the difference of expression levels that are significant (P < 0.05).