Research Paper Volume 11, Issue 23 pp 11504—11519

Resveratrol delays postovulatory aging of mouse oocytes through activating mitophagy

Figure 4. RSV promotes mitophagy in aged oocytes. (A) Electron microscopy results showed the effect of RSV on mitochondrial morphology. Bar = 1 μm. (B) With the treatment of RSV, the autophagy level was detected by counterstaining with LC3 (red), Tom20 (green) and DAPI (blue). Bar = 50 μm. (C) Quantitative analysis of autophagosome numbers in (B). (D) After RSV treatment, the LC3 (including LC3-1 and LC3-2), PINK and Parkin protein expressions were measured by western blot. α-Tubulin was used as a loading control. (E) Quantitative analysis of protein level of PINK1 in (D) and the relative LC3-2/1 level was calculated. Data are presented as means ± S.E.M of three independent experiments. Different lowercase letters represent the difference of expression levels that are significant (P < 0.05).