Research Paper Volume 11, Issue 23 pp 11504—11519

Resveratrol delays postovulatory aging of mouse oocytes through activating mitophagy

Figure 5. RSV-induced mitophagy regulates spindle morphology and CG distribution. (A) The effect of RSV-induced mitophagy on spindle morphology. After mitophagy blocking by CsA, the spindles were stained by anti-α-tubulin (green), and the chromosomes were counterstained with DAPI. Bar = 50 μm. (B) Quantitative analysis of abnormal spindle rates in (A). (C) The effect of RSV-induced mitophagy on CG distribution. After mitophagy blocking by CsA, the CG distribution was determined by staining with anti-PNA (green), and the chromosomes were counterstained with DAPI. Bar = 50 μm. (D) Quantitative analysis of abnormal CG distribution rate in (C). All the experiments were conducted in triplicates. Data are presented as means ± S.E.M of three independent experiments. Different lowercase letters represent the difference of expression levels that are significant (P < 0.05).