Figure 8. The involvement of FoxO3a in RSV-mediated mitophagy during oocyte aging. (A) With the treatment of RSV and CsA, the FoxO3a expression was determined by immunostaining with FoxO3a (red), Tom20 (green) and DAPI (blue). Bar = 50 μm. (B) After the indicated treatment of RSV and CsA, the FoxO3a protein expressions were measured by western blot. α-Tubulin was used as a loading control. (C) Quantitative analysis of FoxO3a fluorescence intensity in (A). (D) Quantitative analysis of FoxO3a protein expression in (B). All experiments were conducted in triplicate. Data are presented as means ± S.E.M of three independent experiments. Different lowercase letters represent the difference of expression levels that are significant (P < 0.05).