Figure 1. NR2F1-AS1 expression in OS tissue samples and cell lines. (A) NR2F1-AS1 expression in 53 pairs of OS tissue samples and adjacent normal tissues was analyzed by RT-qPCR. *P < 0.05 vs. adjacent normal tissues. (B) The expression of NR2F1-AS1 in four human OS cell lines (HOS, U2OS, MG-63, and SAOS-2) and normal osteoblasts (hFOB1.19) was tested by RT-qPCR. *P < 0.05 vs. hFOB1.19 cells. (C) Correlation between NR2F1-AS1 expression and overall survival of patients with OS was determined by Kaplan–Meier analysis; n = 53, P = 0.022.