Research Paper Volume 11, Issue 23 pp 11609—11623

Long noncoding RNA NR2F1-AS1 enhances the malignant properties of osteosarcoma by increasing forkhead box A1 expression via sponging of microRNA-483-3p

Figure 2. NR2F1-AS1 silencing inhibits the proliferation, migration, and invasiveness and promotes the apoptosis of HOS and U2OS cells. (A) Either si-NR2F1-AS1 or si-NC was transfected into HOS and U2OS cells. At 48 h after transfection, RT-qPCR analysis was performed to assess the transfection efficiency. *P < 0.05 vs. group “si-NC.” (B) The CCK-8 assay result showing cell proliferation status under the influence of the NR2F1-AS1 knockdown in HOS and U2OS cells. *P < 0.05 vs. the si-NC group. (C) The apoptotic rate of HOS and U2OS cells after transfection with either si-NR2F1-AS1 or si-NC was detected by means of an Annexin V–FITC Apoptosis Detection Kit. *P < 0.05 vs. group si-NC. (D) Flow cytometry was carried out to examine the cell cycle status of HOS and U2OS cells after transfection with either si-NR2F1-AS1 or si-NC. *P < 0.05 vs. group si-NC. (E, F) Transwell migration and invasion assays quantified the migratory and invasive abilities of HOS and U2OS cells after the transfection of either si-NR2F1-AS1 or si-NC. *P < 0.05 vs. group si-NC.