Research Paper Volume 11, Issue 23 pp 11609—11623

Long noncoding RNA NR2F1-AS1 enhances the malignant properties of osteosarcoma by increasing forkhead box A1 expression via sponging of microRNA-483-3p

Figure 5. Inhibition of miR-483-3p weakens the effects of NR2F1-AS1 knockdown in OS cells. (A) Western blotting analysis of FOXA1 expression in NR2F1-AS1–deficient HOS and U2OS cells. *P < 0.05 vs. the si-NC group. (B) HOS and U2OS cells were transfected with either antagomir-483-3p or antagomir-NC. After 48 h of transfection, total RNA was isolated from the cells and used for quantitation of miR-483-3p. *P < 0.05 vs. group antagomir-NC. (C, D) Si-NR2F1-AS1 along with either antagomir-483-3p or antagomir-NC was introduced into HOS and U2OS cells. The expression levels of miR-483-3p and of the FOXA1 protein in the transfected cells were respectively measured by RT-qPCR and western blotting. *P < 0.05 vs. the si-NC group. #P < 0.05 vs. group si-NR2F1-AS1+antagomir-NC. (EI) The proliferation, apoptotic rate, cell cycle distribution, migration, and invasiveness of the aforementioned cells were determined by the CCK-8 assay, flow cytometry, and transwell migration and invasion assays, respectively. The decrease in cell proliferation, increase in apoptosis, cell cycle arrest, and inhibition of cell migration and invasion were partially reversed by miR-483-3p knockdown. *P < 0.05 vs. group si-NC. #P < 0.05 vs. group si-NR2F1-AS1+antagomir-NC.