Research Paper Volume 11, Issue 23 pp 11609—11623

Long noncoding RNA NR2F1-AS1 enhances the malignant properties of osteosarcoma by increasing forkhead box A1 expression via sponging of microRNA-483-3p

Figure 6. The actions of the NR2F1-AS1 knockdown in OS cells are neutralized by recovery of FOXA1. (A) The efficiency of pc-FOXA1 transfection was assessed via western blotting. *P < 0.05 vs. group pcDNA3.1 (empty-vector control). (BF) HOS and U2OS cells were cotransfected with si-NR2F1-AS1 and either pc-FOXA1 or the empty pcDNA3.1 vector. The CCK-8 assay, flow cytometry, and Transwell migration and invasion assays were conducted to quantitate cellular proliferation, apoptosis, cell cycle distribution, migration, and invasion. *P < 0.05 vs. group si-NC. #P < 0.05 vs. group si-NR2F1-AS1+pcDNA3.1.