Research Paper Volume 11, Issue 23 pp 11609—11623

Long noncoding RNA NR2F1-AS1 enhances the malignant properties of osteosarcoma by increasing forkhead box A1 expression via sponging of microRNA-483-3p

Figure 7. NR2F1-AS1 knockdown restrains the tumor growth of OS cells in vivo. (A) Representative images of tumor xenografts derived from HOS cells transfected with either si-NR2F1-AS1 or si-NC. (B) The volume of tumor xenografts derived from si-NR2F1-AS1–transfected or si-NC–transected HOS cells at 4 weeks postinjection. The tumor growth curves were constructed accordingly. *P < 0.05 vs. the si-NC group. (C) Weights of tumor xenografts. *P < 0.05 vs. group si-NC. (D, E) The expression levels of NR2F1-AS1 and miR-483-3p were determined in the tumor xenografts by RT-qPCR. *P < 0.05 vs. group si-NC. (F) Western blotting was conducted to measure FOXA1 protein amounts in the tumor xenografts from the si-NR2F1-AS1 group and si-NC group. *P < 0.05 vs. the si-NC group.