Figure 3. FHL3 knockdown restrained migration and reversed EMT phenotype in pancreatic cancer cells. (A1 and B1) IF assay of FHL3 in PANC1_NC/KD1-3, BXPC3_NC/KD1-3, which showed KD1 sequence was the most efficient. (A2 and B2) WB assay of FHL3 in PANC1_NC/KD1-3 and BXPC3_NC/KD1-3, which showed the expression level of FHL3 was downregulated more than 80% in PANC1_KD1 and BXPC3_KD1 cells, p<0.001. (C) 48h- transwell assay showed more than 40% decreasing of migration ability of PANC1_KD1 and BXPC3_KD1 cells, as compared with PANC1_NC and BXPC3_NC cells, P<0.01. (D) 72h-wound healing assay showed KD1 cell lines held more blank area as compared with NC cell lines, p<0.05. (E1) WB assay showed FHL3 knockdown reversed the expression level of EMT associated proteins and transcriptional factors, except zeb1.