Figure 10. Immunoprecipitation analysis of nuclear FAK and histone H1. Immunoprecipitation (IP) analysis demonstrated that nuclear FAK associates with H1 histone. IP was carried out with anti-FAK antibody (3285, Cell signaling), followed by immunoblotting with anti-FAK (D2R2E, Cell signaling), anti-pFAK (Y576/577) and anti-H1 antibodies. IP experiments were done on MCF7, HCC1937 and MCF10 cell lines. The images are representative of three independent biological replicates. Panel A shows the levels of FAK in whole and nuclear extracts; Vimentin blot assess that nuclear extracts are not contaminated by cytoplasmic fraction.