Research Paper Volume 11, Issue 23 pp 11722—11755

Histone proteomics reveals novel post-translational modifications in breast cancer

Figure 10. Immunoprecipitation analysis of nuclear FAK and histone H1. Immunoprecipitation (IP) analysis demonstrated that nuclear FAK associates with H1 histone. IP was carried out with anti-FAK antibody (3285, Cell signaling), followed by immunoblotting with anti-FAK (D2R2E, Cell signaling), anti-pFAK (Y576/577) and anti-H1 antibodies. IP experiments were done on MCF7, HCC1937 and MCF10 cell lines. The images are representative of three independent biological replicates. Panel A shows the levels of FAK in whole and nuclear extracts; Vimentin blot assess that nuclear extracts are not contaminated by cytoplasmic fraction.