Figure 4. Tyrosine phosphorylation profiling of histones by 2D Western Blot analysis. 2D TAU Western blot of histone tyrosine phosphorylation pattern (panel A) and densitometry analysis of differentially expressed spots (panel B). The assay was repeated in three independent biological replicates; statistical analysis was done by one-way ANOVA, followed by Dunnett's multiple comparisons test. Differences were considered significant when P≤0.05(*). Data are expressed as mean ± SEM (N =3). Images relative to proteins normalization are provided as Supplementary Figure 2.