Figure 4. HCC subtyping analysis and functional annotation of MRs. (A) Three subgroups were identified by the non-negative matrix factorization (NMF) method in the HCC6 dataset, using consensus hierarchical clustering. (B) Meta-analysis of function annotation based on three subtype-related MR lists. Heatmap shows the top enrichment clusters (gray color indicates lack of significance). (C) Circos plot of MRs’ overlap among the three HCC subtypes. On the inside arc, dark orange color represents the MRs that appear in multiple subtypes and light orange color represents MRs that are unique to that subtype. (D) Enrichment network visualization for biological function from the three HCC subtypes. Nodes are represented by pie charts indicating their associations with each subtype. (E) Protein-protein interaction (PPI) network of MRs.