Research Paper Volume 11, Issue 24 pp 12375—12411

Discovering master regulators in hepatocellular carcinoma: one novel MR, SEC14L2 inhibits cancer cells

Figure 8. SEC14L2 is a potential HCC suppressor. (A) Calculation of dES and survival responses for SEC14L2 in the HCC6 cohort. (B) GO terms enrichment analysis of SEC14L2 regulon. (C) KEGG pathway enrichment analysis of SEC14L2 regulon. (D) Representative target genes of SEC14L2 regulon were significantly up-regulated after SEC14L2 overexpression. (E) SEC14L2 overexpression significantly inhibited cell proliferation (CCK-8 assay) and colony formation in HCC cell lines. Upper and lower panels show corresponding results in MHCC97-H cells. These assays were repeated in Huh7 cells (Supplementary Figure 6). (F) Cell-cycle distribution analysis of MHCC97-H cells overexpressing SEC14L2. (G) Cell migration (wound-healing) assay results. Data represent the average of three independent experiments in duplicate. (H) Effect of SEC14L2 overexpression on tumor xenografts in vivo. Almost total inhibition of tumor growth was observed after subcutaneous implantation of SEC14L2- overexpressing MHCC97-H cells in nude mice. OE, over-expressed; *P < 0.05, **P < 0.01, ***P < 0.001.