Figure 7. MACC1-AS1 overexpression promoted HCC cell proliferation, EMT and invasion through regulating PAX8. (A) The expression of PAX8 was detected in SMMC7721 cell by using qRT-PCR assay. (B) The protein expression of PAX8 was analyzed by using western blotting. (C) Knockdown expression of PAX8 suppressed cell proliferation in MACC1-AS1-overexpressing SMMC7721 cells. (D) Inhibition of PAX8 expression decreased cyclin D1 expression in MACC1-AS1-overexpressing SMMC7721 cells. (E) Knockdown PAX8 expression inhibited vimentin, N-cadherin and snail expression and promoted E-cadherin expression in MACC1-AS1-overexpressing SMMC7721 cell. (F) Knockdown expression of PAX8 decreased cell invasion MACC1-AS1-overexpressing SMMC7721 cell. The relative invasive cells were shown in the right. *p<0.05, **p<0.01 and ***p<0.001.