Research Paper Volume 11, Issue 24 pp 12624—12640

Long noncoding RNA NNT-AS1 enhances the malignant phenotype of bladder cancer by acting as a competing endogenous RNA on microRNA-496 thereby increasing HMGB1 expression

Figure 1. NNT-AS1 is overexpressed in bladder cancer and is associated with poor clinical outcomes. (A) The expression of NNT-AS1 in the 47 pairs of bladder cancer tissue specimens and matched adjacent normal tissues (ANTs) was determined by RT-qPCR. *P < 0.05 vs. the ANTs group. (B) NNT-AS1 levels were measured in four bladder cancer cell lines and a normal bladder immortalized epithelial cell line (SV-HUC-1) by RT-qPCR. *P < 0.05 vs. group SV-HUC-1. (C) Kaplan–Meier plot demonstrating the association between NNT-AS1 expression and overall survival of the patients with bladder cancer. P = 0.0264.