Research Paper Volume 11, Issue 24 pp 12624—12640

Long noncoding RNA NNT-AS1 enhances the malignant phenotype of bladder cancer by acting as a competing endogenous RNA on microRNA-496 thereby increasing HMGB1 expression

Figure 2. Downregulation of NNT-AS1 inhibits the malignant characteristics of bladder cancer cells in vitro. (A) RT-qPCR was carried out to determine the expression of NNT-AS1 in T24 and TCC-SUP cells after either siNNT-AS1 or siNC transfection. *P < 0.05 vs. group siNC. (B, C) The proliferation and apoptosis status of NNT-AS1–depleted T24 and TCC-SUP cells were tested via the CCK-8 assay and flow cytometry. *P < 0.05 vs. group siNC. (D, E) The migration and invasion abilities of T24 and TCC-SUP cells after NNT-AS1 knockdown were evaluated using transwell cell migration and invasion assays. *P < 0.05 vs. the siNC group.