Research Paper Volume 12, Issue 1 pp 156—177

Over-expression of EGFR regulated by RARA contributes to 5-FU resistance in colon cancer

Figure 7. RARA is the transcription factor for EGFR. (A) RARA was upregulated in HT29-R cells compared with parental HT29 cells (n=3). (B) Knockdown of RARA decreased EGFR expression (n=3). (C) Knockdown of RARA blocked autophagy induction in HT29-R cells (n=3). (D) Knockdown of RARA inhibited the conversion of LC3-I to LC3-II in HT29-R cells (n=3, *P < 0.05 versus control). (E) Knockdown of RARA sensitized HT29-R cells to 5-FU treatment (n=3). (F) RARA binds to the EGFR promoter. ChIP assays were performed using anti-RARA antibody or IgG control. Representative data from three individual experiments are shown. (G) Knockdown of RARA reduced EGFR promoter activity as determined by the luciferase assay (n=3).