Figure 3. Effects of IL-12p35 KO on the expression of aging marker-related proteins. (A) The left ventricular p16, p21, p53 and Sirt1 expression levels in each group were investigated by Western blotting analysis; n=10-11 for each group. (B) Cardiac p53 expression was detected by immunofluorescence staining (200x); n=5 for each group. * p<0.05 vs. the young WT group; # p<0.05 vs. the aged WT group.