Figure 6. Effects of IL-12p35 KO on cardiomyocyte apoptosis. (A) Mitochondrial AIF expression and nuclear AIF and Cle-PARP expression were measured by Western blotting analysis. (B) Cardiac AIF expression was detected by immunofluorescence staining (200x). (C) TUNEL staining was performed to mark the apoptotic cells and the positive cell numbers in each group (200x). * p<0.05 vs. the young WT group; # p<0.05 vs. the aged WT group; n=5 for each group.