Figure 1. COL4As expression in hepatocellular carcinoma (HCC). The transcription levels of COL4A1 and COL4A2 were significantly upregulated in preneoplastic lesion (cirrhosis) and HCC tissues compared to normal tissues. (A) Dot plot showing the COL4As mRNA levels in GEO dataset (GSE14323). Normal (n=19): normal liver; Cirrhosis (n=41): HCV cirrhosis without HCC; Cirrhosis HCC (n=17): HCV cirrhosis with HCC; HCV-HCC (n=36): HCC by HCV infection. Data are mean ± SD. *, P < 0.05, **, P < 0.01, ***, P < 0.001 (Student’s t-test). (B) Box plot showing the COL4As mRNA levels in The Cancer Genome Atlas (TCGA) (GEPIA). Normal: n=160; Tumor: n=369. The significance test method was one-way ANOVA, using disease state (Tumor or Normal) as variable for calculating differential expression.