Figure 6. ASPM enhances tumorigenicity of GBM cells in vitro and in vivo. (A–C) RT-qPCR analysis of ASPM expression in stable knockdown U87, U251 and U118 cells. GAPDH was used as an internal reference. sh-NC: shRNA control, sh-1/2: shRNA targeting ASPM. *p < 0.05 versus control. (D–F) The growth rate of U87, U251 and U118 cells with stable ASPM knockdown was monitored by the RTCA-MP system. (G–I) Colony formation assay was performed using U87, U251 and U118 cells with stable ASPM knockdown. Representative images (up) and quantification analysis (down) are shown, and the data shown are the mean ± SD (n = 3). ***p < 0.001 versus control. (J–L) ASPM knockdown inhibited tumour growth in the U251 xenograft assay. Photos of xenograft tumours (J), tumour volume (K) and tumour weight (L) are shown. sh-NC, n = 10; sh-1, n = 10. **p < 0.01, ***p < 0.001 versus control.