Research Paper Volume 12, Issue 1 pp 318—339

Circ-EPB41L5 regulates the host gene EPB41L5 via sponging miR-19a to repress glioblastoma tumorigenesis


Figure 2. Suppression effects of circ-EPB41L5 on glioma cells. (A) qRT-PCR detected circ-EPB41L5 expression in normal human astrocytes (NHAs) and glioma cells (U87, T98, A172, LN229, U251, U373). (B) qRT-PCR tested the efficiency of circ-EPB41L5sh plasmids in LN229 cells. qRT-PCR tested the efficiency of circ-EPB41L5 overexpression plasmids in U87 and U251 cells. (C) CCK-8 assays determined the cell viability after stable transfection with vector, circ-EPB41L5sh, or circ-EPB41L5 overexpression plasmid in U87, U251, and LN229 cells. (D) Colony formation assays detected the cell proliferation ability; representative images are presented. Colony formation rates were normalized to the vector group. Scale bars: 4 mm. (E) Transwell assays measured the migration and invasion abilities of glioma cells; representative images are presented. Scale bars: 100 μm. The data are the means±SEM of three experiments, *P<0.05, **P<0.01.