Research Paper Volume 12, Issue 1 pp 318—339

Circ-EPB41L5 regulates the host gene EPB41L5 via sponging miR-19a to repress glioblastoma tumorigenesis

Figure 4. circ-EPB41L5 suppressed glioma cell proliferation, migration, and invasion. (A) The expression level of EPB41L5 in glioma from the Rembrandt dataset. The level of EPB41L5 was decreased in glioblastoma. (B) Expression level of EPB41L5 in different subtypes of glioblastoma from TCGA dataset. The level of EPB41L5 was the lowest in mesenchymal. (C, D) Kaplan–Meier survival analysis of glioblastoma patients from the Rembrandt and TCGA datasets. Low expression of EPB41L5 indicated poor prognosis. (EG) Knockdown of EPB41L5 in U87 and U251 promoted proliferation, colony formation, migration, and invasion abilities of glioma cells. Overexpression of circ-EPB41L5 impaired the oncogenic effects of EPB41L5 knockdown. Scale bars: 4 mm in colony formation assays. Scale bars: 100 μm in transwell assays. (H) Quantification of colony formation rates and relative migration or invasion cell number. The data are means±SEM of three experiments, *P<0.05, **P<0.01.