Research Paper Volume 12, Issue 1 pp 318—339

Circ-EPB41L5 regulates the host gene EPB41L5 via sponging miR-19a to repress glioblastoma tumorigenesis


Figure 6. Overexpression circ-EPB41L5 inhibited tumorigenesis of glioblastoma in vivo. Overexpression circ-EPB41L5 inhibited the growth of brain xenograft tumors of U251 glioma cells. (A) Representative bioluminescence images and H&E stained images; scale bars: 1 mm. (B) Quantification of the bioluminescence activity. Data were from five mice per group. (C) Quantification of tumor size. Data were from five mice per group. (D) Kaplan–Meier survival analysis of mice implanted with U87 cells stably overexpressing circ-EPB41L5 or EPB41L5sh plasmids. Data were from five mice per group. (E) WB assays measured the expression of EPB41L5, RhoC, p-AKT, and total AKT in treated U87 and U251 cells. (F) Schematic representation of circ-EPB41L5-miR-19a-EPB41L5-AKT regulatory axis in glioblastoma. *P<0.05, **P<0.01.