Figure 5. Both EBV-miR-BART13-3p overexpression and ABI2 knockdown propels EMT through c-JUN/SLUG signaling. (A) mRNA expression of common EMT-TFs regulators (SNAIL, SLUG, CTNNNB1, TWIST1, FOXC2, ZEB1 and ZEB2) were detected by RT-qPCR in CNE1 and S26 cells. (B) RT-qPCR assay showed the mRNA expression of c-JUN was up-regulated in NPC cells with high levels of BART13-3p. (C) Western blotting assay showed the protein expression of c-JUN and SLUG was up-regulated by BART13-3p. (D, E) Knockdown of ABI2 by siRNA increased both mRNA and protein expression of c-JUN and SLUG. Error bars represent SEM. (*P<0.05; **P<0.01; ***P<0.001; N.S. P>0.05).