Research Paper Volume 12, Issue 1 pp 340—358

Downregulation of ABI2 expression by EBV-miR-BART13-3p induces epithelial-mesenchymal transition of nasopharyngeal carcinoma cells through upregulation of c-JUN/SLUG signaling

Figure 6. Restitution of ABI2 rescues the phenotypes generated by EBV-miR-BART13-3p. (A) Restoration of ABI2 reversed the morphological changes of NPC cells induced by BART13. (B) Restitution of ABI2 increased the protein expression of E-cadherin and reduced Vimentin expression in NPC cells with elevated level of BART13-3p measured by western blot. Meanwhile, c-JUN/SLUG signaling was also reversed. (C, D) Transwell migration assay and invasion assay validated that reconstitution of ABI2 reduced the cell migration and invasion of BART13-3p up-regulated NPC cells. (E) A schematic of BART13-3p inducing increased c-JUN/SLUG signaling and leading to EMT of NPC cells mediated by ABI2. Scale bar, 100μm. Error bars represent SEM. (*P<0.05; **P<0.01; ***P<0.001).