Figure 1. High expression of VPS9D1-AS1 in NSCLC indicating poor prognosis in NSCLC patients. (A) RT-qPCR analysis of VPS9D1-AS1 expression in 51 pairs of NSCLC samples and corresponding normal lung tissues. *P < 0.05 vs. normal lung tissues. (B) Relationship between VPS9D1-AS1 expression and overall survival of patients with NSCLC analyzed by the Kaplan–Meier method and log-rank test. P = 0.030. (C) Determination of VPS9D1-AS1 expression by RT-qPCR in total RNA from five NSCLC cell lines (H522, H460, H1299, A549, and SK-MES-1) and one normal nontumorigenic bronchial epithelium cell line (BEAS-2B). *P < 0.05 vs. BEAS-2B cells.