Figure 5. SP-2509 and JQ1 increase cell invasion in high AR-expressing cells (LNCaP, LAPC4 and 22Rv1) but decrease cell invasion in low/negative AR-expressing cells (C4-2, PC3 and DU145). (A) Cell morphology for LNCaP and LAPC4 cells was shown after treatment with 1μM SP-2509 or JQ1 alone, or in combination for 24 h. (B) Graphic representation of the fold change in invading cells with statistical significance presented for AR-positive and AR-negative cell types. (C) The cell invasion analysis was performed in AR knockdown LNCaP and 22Rv1 cells, or AR expressing PC3 cells with treatments as described in (A). Representative images are shown (left) and graphic representation of the fold changes in the invading cells with statistical significance was indicated (right). Statistical differences are determined by ANOVA with: * indicates P < 0.05; ** indicates by P < 0.01; *** indicates P < 0.001. Scare bar, 200μm.