Research Paper Volume 12, Issue 5 pp 4067—4081

HIF-1α/microRNA-128-3p axis protects hippocampal neurons from apoptosis via the Axin1-mediated Wnt/β-catenin signaling pathway in Parkinson’s disease models

Figure 1. The expression pattern of HIF-1α/miR-128-3p/AXIN1 and the Wnt/β-catenin signaling pathway-related proteins (β-catenin, cyclin D1 and c-met) in hippocampal neurons of normal mice (n = 10) and the MPTP-lesioned mouse model of PD (n = 30). (A) The miR-128-3p expression and mRNA levels of Hif1a, Axin1, Ctnnb1, Ccnd1, and Met determined by RT-qPCR. (B) The protein levels of HIF-1α, AXIN1, β-catenin, cyclin D1, and c-met normalized to β-actin as determined by western blot analysis. (C) The localization of β-catenin protein in hippocampal tissues detected by the immunofluorescence assay (scale bar = 25 μm). (D) The hippocampal neuron apoptosis identified by flow cytometry. (E) The ultrastructure of hippocampal neurons through electron microscopy. * p < 0.05 vs. the control group (primary hippocampal neurons of normal mice).