Research Paper Volume 12, Issue 1 pp 543—570

Activation of α7 nAChR by PNU-282987 improves synaptic and cognitive functions through restoring the expression of synaptic-associated proteins and the CaM-CaMKII-CREB signaling pathway

Figure 11. Activation of α7 nAChR activates the CaM-CaMKII-CREB signaling pathway in the hippocampus of APP/PS1_DT mice. The x-axis indicates the WT mice (control); WT mice treated with PNU (WP); APP/PS1_DT mice (APP/PS1); and APP/PS1_DT mice treated with PNU (AP). The y-axes indicate the relative mRNA and protein levels as a percentage of the control. Compared with the control group, the transcription levels of CaM (A), CaMKIIα (C) and CREB (E) were significantly decreased in the hippocampus of APP/PS1_DT mice. The protein expression levels of CaM (B), CaMKIIα (D), CREB (F), p-CaMKIIα (G) and p-CREB (H) were also significantly reduced. Moreover, the p-CaMKIIα/CaMKIIα (I) and p-CREB/CREB (J) ratios were significantly reduced. The protein expression levels of CaM, CaMKII and CREB in the hippocampus of APP/PS1_DT mice were significantly increased after PNU treatment (except CREB and CaMKIIα/CaMKIIα in 6-month mice in the AP group). Data are presented as the mean ± standard deviation. *P<0.05, **P<0.01 vs. control group; #P<0.05, ##P<0.01 vs. APP/PS1 group.