Research Paper Volume 12, Issue 1 pp 543—570

Activation of α7 nAChR by PNU-282987 improves synaptic and cognitive functions through restoring the expression of synaptic-associated proteins and the CaM-CaMKII-CREB signaling pathway

Figure 4. Activation of α7 nAChR promotes the expression of synaptic-associated proteins in Aβ oligomer-treated neurons. The x-axis labels are the neurons isolated from the WT rat (control), the WT neuron cells treated with PNU (PNU), the WT neuron cells treated with Aβ (Aβ) and the WT neuron cells treated with PNU and Aβ (PNU+Aβ). The y-axis indicates the relative level of mRNA or protein (% of control). Detection of SYN (A) mRNA and (B) protein; PSD95 (C) mRNA and (D) protein; SNAP25 (E) mRNA and (F) protein; DYN1 (G) mRNA and (H) protein; AP180 (I) mRNA and (J) protein. The relative level in each group was measured by RT-qPCR and western blot analysis, and β-actin was used as an internal control. The results demonstrated that the protein expression levels of SYN, PSD95, SNAP25, DYN1 and AP180 were significantly decreased in Aβ oligomer-treated neurons, and this decrease was partially reversed by PNU treatment. Data are presented as the mean ± standard deviation. *P<0.05, **P<0.01 vs. control group; #P<0.05, ##P<0.01 vs. Aβ.