Research Paper Volume 12, Issue 1 pp 543—570

Activation of α7 nAChR by PNU-282987 improves synaptic and cognitive functions through restoring the expression of synaptic-associated proteins and the CaM-CaMKII-CREB signaling pathway

Figure 7. Activation of α7 nAChR attenuates the expression of SYN in the hippocampus of APP/PS1_DT mice. (A) Hippocampus cells isolated from WT mice (Control); (B) Controls treated with PNU (WP); (C) 6-month-old APP/PS1_DT mice; (D) 6-month-old APP/PS1_DT mice treated with PNU; (E) 10-month-old APP /PS1_DT mice and (F) 10-month-old APP/PS1 mice treated with PNU. (G) The IOD of SYN in the hippocampus DG area, which was collected from 6-month-old mice. (H) The IOD of SYN in the hippocampus DG area, which was collected from 10-month-old mice. SYN, Aβ and nucleus are labeled in green, red and blue, respectively. Magnification, x200. Compared with the (A) control group, the expression level of SYN was significantly decreased in the hippocampus DG area of (C) 6- and (E) 10- month-old APP/PS1_DT mice. (D and F) The Aβ distribution and SYN expression were negatively correlated, and the decrease of SYN could be partially reversed by PNU treatment. Data are presented as the mean ± standard deviation. *P<0.05, **P<0.01 vs. control group; #P<0.05, ##P<0.01 vs. APP/PS1 group.