Figure 1. Cortical expression profile of intracellular cholesterol carriers and sensors/regulators. (A) Relative mRNA levels of NPC1, StARD3, StARD4, and StARD1 in human cortex from AD (n=5), DS (n=5), and control (n=6) subjects. Transcripts levels were normalized with respect to controls using -actin. (*) p<0.05; (**) p<0.01. (B) Immunoblotting of NPC1, StARD1, StARD3/MLN64, mSREBP2 and INSIG-1 of total protein extracts (90 μg/lane) from human cortex from AD (n=5), DS (n=5), and control (n=6) donors. (C) Protein levels quantified by densitometry and normalized using -actin as housekeeping followed by normalization with control group. (*) p<0.05; (**) p<0.01.