Figure 3. The expression of inflammatory cytokines and fibrotic factors are reduced in STZ-induced diabetic rats. (A) The kidney tissues were fixed in formalin and then subjected to immunofluorescence detection of TNF-α (arrow heads pointing to dark-brown dots indicating TNF-α expression). n = 5 per group, (B) IL6 protein level (C) MCP1 protein level (D) TGF-β mRNA level, (E) TGF-β protein level with a representative blot, (F) Fibronectin (FN) mRNA level in kidney tissues. Data are shown as the means ± SEM *p < 0.05 vs. WT-CON; #p < 0.05 vs. WT-STZ. n = 5–8 per group.