Research Paper Volume 12, Issue 1 pp 593—610

Attenuation of diabetic kidney injury in DPP4-deficient rats; role of GLP-1 on the suppression of AGE formation by inducing glyoxalase 1


Figure 4. Increased of AGE formation, RAGE and GLO-1 expression are inhibited in the kidney of DPP4 deficient diabetic rats. Kidney samples were collected at 42 days, since over 300 mg/dL of blood glucose after STZ injection as described in Materials and Methods section. AGEs formation was evaluated using antibody against AGEs in the kidney section. Brown color indicates AGEs formation in staining. (A) AGEs formation, (B) RAGE protein level with representative blot (C) GLO1 mRNA level (D) GLO-1 protein level with a representative blot in tissues. WT-CON: wild-type control, WT-STZ: wild-type-STZ, DPP4-def-CON: DPP4-deficient control, DPP4-def-STZ: DPP4-deficient-STZ. Data are shown as the means ± SEM. *p < 0.05 and WT-CON, #p < 0.05 and WT-STZ, n = 7–8 per group.