Research Paper Volume 12, Issue 1 pp 593—610

Attenuation of diabetic kidney injury in DPP4-deficient rats; role of GLP-1 on the suppression of AGE formation by inducing glyoxalase 1

Figure 8. AGEs formation and inflammatory cytokines are further increased in the knockdown of GLO-1. Rat mesangial cells were transfected either with siRNA control or siRNA GLO-1 and, then, treated with 0.75 mM MGO and 10 nM Ex-4 for 4 h. (A) siRNA GLO-1 transfection efficacy. (B) AGEs formation level in the knockdown of GLO-1 and mRNA expression levels of inflammatory cytokines including (C) TNF-α (D) MCP-1, and (E) IL-6. Data are shown as the means ± SEM. *p < 0.05 vs. siRNA CON, #p < 0.05 vs. siRNA CON + MGO, p < 0.05 vs. siRNA GLO-1 + CON, p < 0.05 vs. siRNA CON + MGO + Ex-4. n = 5–6.