Priority Research Paper Volume 12, Issue 1 pp 8—34

Resveratrol targets PD-L1 glycosylation and dimerization to enhance antitumor T-cell immunity

Figure 2. Resveratrol alters PD-L1 N-glycosylation in a SIRT1-, AMPK-, and GSK3β-independent manner. (A) Representative immunoblot of SIRT1, acetyl-p53 (Lys382), and PD-L1 in JIMT-1 cells cultured with or without RSV in the absence or presence of the AMPK inhibitor compound C, the SIRT1 inhibitor EX-527, and the GSK3β inhibitors AR-18 and LiCl. (B) Schematic representation of the biosynthesis and processing PD-1 N-linked glycosylation pathway, showing the sites of action of well-known glycoprotein-processing enzymes inhibitors. RSV is proposed to operate as a direct inhibitor of GAA and/or α-Man I enzymatic activities.