Research Paper Volume 12, Issue 1 pp 650—671

Spermidine alleviates cardiac aging by improving mitochondrial biogenesis and function

Figure 7. Deletion of PGC-1α abrogates SPD-mediated enhancement of mitochondrial biogenesis. (A) Western blot analysis of PGC-1α expression in H9C2 cells transfected with siRNA against PGC-1α (si) or control (scrambled) siRNA (ci). (B) Western blot and quantification analysis of PGC-1α, NRF1, NRF2, and TFAM expression in transfected H9C2 cells in the absence (ci and si) or presence of SPD (ci + SPD and si + SPD). GAPDH served as loading control (n = 4). * P < 0.05 vs. Scramble Control.