Research Paper Volume 12, Issue 1 pp 844—865

Surgery/Anesthesia disturbs mitochondrial fission/fusion dynamics in the brain of aged mice with postoperative delirium

Figure 1. Diagram of the experimental design. The mice underwent behavioral tests at 24 hours before (baseline) and at 6, 9, and 24 hours after the Surgery/Anesthesia. Within each group, separate cohorts were subjected to assessments at each time point (n = 9 per cohort). Mice were sacrificed immediately after the Surgery/Anesthesia and at 6, 9, and 24 hours postoperatively. The hippocampal and prefrontal cortex tissues were harvested for analysis of DISC1, Drp1, Mfn2, SOD, ROS, ATP, MMP, BDNF, and PSD-95 levels (n = 6 per cohort). Mice were anesthetized and transcardially perfused with ice-cold phosphate-buffered saline (PBS) followed by paraformaldehyde and glutaraldehyde; then, hippocampal and prefrontal cortex tissues were collected and stored in the same fixative for electron microscopy analysis immediately after the Surgery/Anesthesia and at 24 hours postoperatively (n = 3 per cohort). DISC1, disrupted in schizophrenia 1. Drp1, dynamin-related protein 1. Mfn2, mitofusin 2. SOD, superoxide dismutase. ROS, reactive oxygen species. ATP, adenosine triphosphate. MMP, mitochondrial membrane potential. PSD-95, postsynaptic density protein 95. BDNF, brain-derived neurotrophic factor.