Figure 10. Surgery/Anesthesia decreased PSD-95 and BDNF expression in the hippocampus and prefrontal cortex of aged mice at postoperative 0, 6, 9, and 24 hours. The expression of PSD-95 and BDNF was estimated using western blotting analysis of fresh homogenates of hippocampal and prefrontal cortex tissues obtained at 0, 6, 9, and 24 hours after Surgery/Anesthesia or control treatment. Immediately after Surgery/Anesthesia, there were no significant differences in the PSD-95 expression in the hippocampus (A and C) and prefrontal cortex (B and D) between the mice in the Surgery/Anesthesia group and control group. However, the level of PSD-95 in the hippocampus (A and C) and prefrontal cortex (B and D) of mice in the Surgery/Anesthesia group decreased significantly compared to that in control mice at postoperative 6, 9, and 24 hours. Additionally, Surgery/Anesthesia decreased the BDNF expression in the hippocampus (E and G) and the prefrontal cortex tissues (F and H) at 24 but not 0, 6, or 9 hours postoperatively when compared to the control condition in mice. The data are plotted as the mean ± standard error of the mean for each group (n = 6). *p < 0.05 and **p < 0.01, compared to control.